Adapting the laser-induced fluorescence detection setup of the standard capillary electrophoresis equipment to achieve high-sensitivity detection of 2-aminoacridone labeled oligosaccharides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00617403" target="_blank" >RIV/68081715:_____/25:00617403 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00141057
Result on the web
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70112" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70112</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.70112" target="_blank" >10.1002/jssc.70112</a>
Alternative languages
Result language
angličtina
Original language name
Adapting the laser-induced fluorescence detection setup of the standard capillary electrophoresis equipment to achieve high-sensitivity detection of 2-aminoacridone labeled oligosaccharides
Original language description
The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides. An external solid-state laser with a wavelength of 405 nm was connected to the commercial capillary electrophoresis instrument via a simple 3D-printed laser-to-light-guide adapter, and different optical filter setups were compared based on the signal-to-noise ratio. The optimized setup provided detection limits as low as 0.27 to 0.34 nM, corresponding to injection of 3.4 to 4.6 attomoles of 2-aminoacridone labeled oligosaccharides. These findings show that the optimized laser and filter configuration can enhance the sensitivity of electrophoretic separation by several orders of magnitude. In addition, the presented setup can be utilized as a guide for coupling different lasers to the commercial instrument.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
1615-9314
Volume of the periodical
48
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
e70112
UT code for WoS article
001445198900001
EID of the result in the Scopus database
2-s2.0-105000445466