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Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F15%3A00446563" target="_blank" >RIV/68081731:_____/15:00446563 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.3109/10242422.2015.1053470" target="_blank" >http://dx.doi.org/10.3109/10242422.2015.1053470</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3109/10242422.2015.1053470" target="_blank" >10.3109/10242422.2015.1053470</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules

  • Original language description

    A high-performance biocatalyst in the form of encapsulated cells of Gluconobacter oxydans have been developed for production of phenylacetic acid (PAA) as a natural flavor component. Polyelectrolyte complex (PEC) capsules consisting of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl2, and NaCl were used for highly controlled and mild encapsulation of cells. Utilization of encapsulated G. oxydans cells was a significant improvement on existing data on operational stability of cellsand cumulative product concentration during biocatalytic production of PAA from 2-phenylethanol. Concerning operational stability, encapsulated cells were active over 12 cycles with a high biotransformation rate, while free cells were inactive after 7 cycles of use. The biocatalytic properties of encapsulated G. oxydans were tested in a bubble column reactor over 7 days with a final cumulative product concentration of 25 g/L. High cell viability (90%) was observed within PEC capsules by

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    JA - Electronics and optoelectronics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA14-22777S" target="_blank" >GA14-22777S: The study of electron-gas interactions in conditions of pressure gradient of low energy environmental scanning electron microscope.</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biocatalysis and Biotransformation

  • ISSN

    1024-2422

  • e-ISSN

  • Volume of the periodical

    33

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    10

  • Pages from-to

    111-120

  • UT code for WoS article

    000359802000006

  • EID of the result in the Scopus database

    2-s2.0-84938328523