Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00499922" target="_blank" >RIV/68081731:_____/18:00499922 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41377-018-0111-0" target="_blank" >http://dx.doi.org/10.1038/s41377-018-0111-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41377-018-0111-0" target="_blank" >10.1038/s41377-018-0111-0</a>

Result language
angličtina
Original language name
Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber
Original language description
Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
<a href="/en/project/EF15_003%2F0000476" target="_blank" >EF15_003/0000476: Holographic endoscopy for in vivo applications</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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