Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00638398" target="_blank" >RIV/68081731:_____/25:00638398 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/25:00141772 RIV/00159816:_____/25:00082346

Result on the web

<a href="https://www.mdpi.com/1424-8220/25/15/4629" target="_blank" >https://www.mdpi.com/1424-8220/25/15/4629</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s25154629" target="_blank" >10.3390/s25154629</a>

Result language

angličtina

Original language name

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

Original language description

Highlights: What are the main findings? Adenine was detected as a stress marker in five bacterial strains. Stable Au and Ag NPs enabled SERS-based analysis of secreted bacterial metabolites. What is the implication of the main findings? Label-free SERS detects metabolic stress responses in bacteria cost-effectively. Gold NPs enable long-term, reproducible Raman-based bacterial stress detection. Abstract: This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor-demineralized water-inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

<a href="/en/project/LM2023050" target="_blank" >LM2023050: National Infrastructure for Biological and Medical Imaging</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

1424-8220

Volume of the periodical

25

Issue of the periodical within the volume

15

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

4629

UT code for WoS article

001549756200001

EID of the result in the Scopus database

2-s2.0-105013297439