Suppression of IL-10 production by activated B cells via a cell contact-dependent cyclooxygenase-2 pathway upregulated in IFN-γ-treated mesenchymal stem cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00450580" target="_blank" >RIV/68378041:_____/16:00450580 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10315743
Result on the web
<a href="http://dx.doi.org/10.1016/j.imbio.2015.09.017" target="_blank" >http://dx.doi.org/10.1016/j.imbio.2015.09.017</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.imbio.2015.09.017" target="_blank" >10.1016/j.imbio.2015.09.017</a>
Alternative languages
Result language
angličtina
Original language name
Suppression of IL-10 production by activated B cells via a cell contact-dependent cyclooxygenase-2 pathway upregulated in IFN-γ-treated mesenchymal stem cells
Original language description
The immunoregulatory properties of mesenchymal stem cells (MSCs) have been well documented in various models in vitro and in vivo. Furthermore, a population of regulatory B cells (Bregs) that produce relatively high concentrations of IL-10 has been recently described. To study the relationship between MSCs and Bregs, we analyzed the effects of MSCs on IL-10 production by lipopolysaccharide (LPS)-activated mouse B cells. The production of IL-10 by B cells remained preserved in the presence of MSCs and was even significantly enhanced by IFN-gamma. However, the production of IL-10 was strongly suppressed in cultures containing MSCs and IFN-gamma. Preincubation of MSCs, but not of B cells, with IFN-gamma induced the suppression of IL-10 secretion in cultures containing MSCs and B cells. The supernatants from IFN-gamma-treated MSCs had no inhibitory effect, and the suppression of IL-10 production was abrogated if the MSCs and B cells were separated in a transwell system. Analysis of the gene expression of IFN-gamma- or IFN-gamma and LPS-treated MSCs revealed a strong upregulation of genes for indoleamine-2,3-dioxygenase (IM), cyclooxygenase-2 (Cox-2) and programmed cell death-ligand 1 (PD-L1). While the inhibition of IDO activity or the inclusion of the neutralization monoclonal antibody anti-PD-L1 did not abrogate the suppression, indomethacin, an inhibitor of Cox-2, completely inhibited the MSC-mediated suppression of IL-10 production. Accordingly, the production of IL-10 by B cells was inhibited by exogenous prostaglandin E-2. The results thus suggest that IFN-gamma-treated MSCs strongly inhibit IL-10 production by activated B cells by a mechanism requiring cell contact and involving the Cox-2 pathway. (C) 2015 Elsevier GmbH. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Immunobiology
ISSN
0171-2985
e-ISSN
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Volume of the periodical
221
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
129-136
UT code for WoS article
000369046700001
EID of the result in the Scopus database
2-s2.0-84989790738