Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00477250" target="_blank" >RIV/68378041:_____/17:00477250 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21220/17:00317951 RIV/68407700:21720/17:00317951 RIV/00216208:11130/17:10373781 RIV/00216208:11310/17:10373781
Result on the web
<a href="http://dx.doi.org/10.1111/cpr.12357" target="_blank" >http://dx.doi.org/10.1111/cpr.12357</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/cpr.12357" target="_blank" >10.1111/cpr.12357</a>
Alternative languages
Result language
angličtina
Original language name
Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides
Original language description
Objectives: Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III, bone morphogenetic protein 7 (BMP-7) and BMP-2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells (pMSCs). nnMaterials and methods: pMSCs were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement. Analysis of pMSCs cultures included measurement of the metabolic activity and proliferation, immunofluorescence staining and also qPCR. nnResults: Results showed no detrimental effect of the bioactive peptides to cultured pMSCs. Based on qPCR analysis, the bioactive peptides are specific for osteogenic differentiation with no detectable expression of collagen II. Our results further indicate that peptide derived from BMP-2 protein promoted the expression of mRNA for osteocalcin (OCN) and collagen I significantly compared to control groups and also supported deposition of OCN as observed by immunostaining method. nnConclusion: The data suggest that bioactive peptide with an amino acid sequence of KIPKASSVPTELSAISTLYL derived from BMP-2 protein was the most potent for triggering osteogenic differentiation of pMSCs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cell Proliferation
ISSN
0960-7722
e-ISSN
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Volume of the periodical
50
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
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UT code for WoS article
000407139200013
EID of the result in the Scopus database
2-s2.0-85024127452