Therapeutic effect of molecular hydrogen in corneal UVB-induced oxidative stress and corneal photodamage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00487529" target="_blank" >RIV/68378041:_____/17:00487529 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10367084

Result on the web

<a href="http://dx.doi.org/10.1038/s41598-017-18334-6" target="_blank" >http://dx.doi.org/10.1038/s41598-017-18334-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-18334-6" target="_blank" >10.1038/s41598-017-18334-6</a>

Result language

angličtina

Original language name

Therapeutic effect of molecular hydrogen in corneal UVB-induced oxidative stress and corneal photodamage

Original language description

The aim of this study is to examine whether molecular hydrogen (H-2) is able to reduce oxidative stress after corneal damage induced by UVB irradiation. We previously found that UVB irradiation of the cornea caused the imbalance between the antioxidant and prooxidant enzymes in the corneal epithelium, followed by the imbalance between metalloproteinases and their physiological inhibitors (imbalances in favour of prooxidants and metalloproteinases) contributing to oxidative stress and development of the intracorneal inflammation. Here we investigate the effect of H-2 dissolved in PBS in the concentration 0.5 ppm wt/vol, applied on rabbit corneas during UVB irradiation and healing (UVB doses 1.01 J/cm(2) once daily for four days). Some irradiated corneas remained untreated or buffer treated. In these corneas the oxidative stress appeared, followed by the excessive inflammation. Malondiladehyde and peroxynitrite expressions were present. The corneas healed with scar formation and neovascularization. In contrast, in H-2 treated irradiated corneas oxidative stress was suppressed and malondiladehyde and peroxynitrite expressions were absent. The corneas healed with the restoration of transparency. The study provides the first evidence of the role of H-2 in prevention of oxidative and nitrosative stress in UVB irradiated corneas, which may represent a novel prophylactic approach to corneal photodamage.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30207 - Ophthalmology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

dec

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

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UT code for WoS article

000418562100057

EID of the result in the Scopus database

2-s2.0-85038887353