Characterisation of mesenchymal stem cells from patients with amyotrophic lateral sclerosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F18%3A00492621" target="_blank" >RIV/68378041:_____/18:00492621 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/18:10386792
Result on the web
<a href="http://dx.doi.org/10.1136/jclinpath-2017-204681" target="_blank" >http://dx.doi.org/10.1136/jclinpath-2017-204681</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1136/jclinpath-2017-204681" target="_blank" >10.1136/jclinpath-2017-204681</a>
Alternative languages
Result language
angličtina
Original language name
Characterisation of mesenchymal stem cells from patients with amyotrophic lateral sclerosis
Original language description
Aims Mesenchymal stem cells (MSCs) have recently been tested in clinical trials to treat severe diseases, including amyotrophic lateral sclerosis (ALS). Since autologous MSCs are frequently used for therapy, we aimed to evaluate the possible influence of the disease on characteristics and function of these cells. Methods MSCs were isolated from the bone marrow of patients with ALS and compared with MSCs from healthy controls (HC). The cells were tested for phenotype, growth properties, differentiation ability, metabolic activity, secretory potential, expression of genes for immunomodulatory molecules and for the ability to regulate proliferation of mitogen-stimulated peripheral blood leucocytes. MSCs from patients with ALS and HC were either unstimulated or treated with proinflammatory cytokines for 24 hours before testing. Results MSCs isolated from patients with ALS have a higher differentiation potential into adipocytes, express elevated levels of mRNA for interleukin-6, but produce less hepatocyte growth factor than MSCs from HC. On the other hand, there were no significant differences between MSCs from patients with ALS and HC in the expression of phenotypic markers, growth properties, metabolic activity, osteogenic differentiation potential and immunoregulatory properties. Conclusions The results suggest that, in spite of some differences in cytokine production, MSCs from patients with ALS can be useful as autologous cells in therapy of ALS.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Pathology
ISSN
0021-9746
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
735-742
UT code for WoS article
000442467400012
EID of the result in the Scopus database
2-s2.0-85049186661