Analysis of Ca2+ Signaling Mechanisms - Our Experience on the Intercellular Communication in Muscle Remodeling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518849" target="_blank" >RIV/68378041:_____/19:00518849 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/19:10396566 RIV/00216208:11140/19:10396566
Result on the web
<a href="http://www.biomed.cas.cz/physiolres/pdf/2019/68_325.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/2019/68_325.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934082" target="_blank" >10.33549/physiolres.934082</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of Ca2+ Signaling Mechanisms - Our Experience on the Intercellular Communication in Muscle Remodeling
Original language description
The aim of this study was to evaluate cell diversity by considering how Ca2+ signaling has been adapted in skeletal muscle cell function. We characterized single C2C12 myoblasts through intracellular Ca2+ signaling kinetics after exposure to specific drugs and calcium blockers using fast fluorescence microspectrofluorimetry followed by ATP effect analysis, which confirmed the expression of functional purinergic adenosine and P2 receptors. Further, we found that glutamate sensitivity of C2C12 cells was mediated by ionotropic glutamate receptors, on the other hand, most cells were responsive to cyclopiazonic acid, which inhibits the sarco-endoplasmic reticulum Ca2+-ATPase pump. These results suggest that C2C12 cells possess functional L- and P/Q-type voltage-operated Ca2+ channels, ryanodine receptors and functional sarcoplasmic reticulum Ca2+ stores (typical for muscle cells), adenosine and P2 purinergic receptors, as well as ionotropic glutamate receptors. The evaluation of intracellular Ca2+ signaling is a promising approach towards a better understanding and control of the physiopathological properties of myogenic cells that could be used as a predictive factor in the selection of optimal cells for scaffold recellularization or for tissue engineered constructs used in stem cell therapy.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
<a href="/en/project/GA15-09161S" target="_blank" >GA15-09161S: Muscle remodelling on the basis of extracellular matrix seeded with functionally characterized cells.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
4
Pages from-to
325-328
UT code for WoS article
000465948800019
EID of the result in the Scopus database
2-s2.0-85065487824