NANODIAMONDS ENCAPSULATED IN NANOFIBERS AS A NEW STRATEGY FOR MICRORNA-BASED THERAPY OF PROSTATE CANCER

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00522949" target="_blank" >RIV/68378041:_____/19:00522949 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/19:00522949 RIV/68407700:21720/19:00340546

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

NANODIAMONDS ENCAPSULATED IN NANOFIBERS AS A NEW STRATEGY FOR MICRORNA-BASED THERAPY OF PROSTATE CANCER

Original language description

Given the miRNA-34 family role as tumor suppressor in cancer, it was showed that using miRNA-34a as a new anticancer agent has a great therapeutic potential. Unfortunately, biopharmaceutical issues, rapid degradation in biological fluids, poor uptake into cells and not specific distribution into the body, prevent the use of wildtype non-coding RNA by systemic administration. To overcome these limits, in our previous study we developed a new therapeutic concept based on nanotechnology delivery of miRNA-34a into prostate cancer using fluorescent nanodiamond particles coated with polyethylenimine. In the current study, in order to achieve a more conservative, stable and controlled release of miRNA-34a, we designed a system based on nanodimaonds- miRNA34a complexes encapsulated into nanofibers. We successfully encapsulate nanodiamonds into fibers of polycaprolactione, a biodegradable polymer with a slow degradation rate and a high cellular adhesion, using an electropsinning process. We cultivated prostate cancer cells (PC3 and DU145) on nanofibers scaffolds and we analyzed the presence of nanodiamonds in cells using confocal microscopy. Moreover, in these prostate cancer cell lines we observed a replacement of miRNA-34a via real-time PCR and a decreased viability testing the metabolic activity. Our preliminar results suggest a higher increased and stable mid-term replacement of oncosuppressor miRNA-34a in prostate cancer when the nanodiamonds-miRNA complexes are delivered encapsulating them in nanofibers. Therefore we provided an effective strategy for cancer therapy ready to be tested for in vivo experiments.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30404 - Biomaterials (as related to medical implants, devices, sensors)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application

ISBN

978-808729489-5

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

348-353

Publisher name

Tanger Ltd.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 17, 2018

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000513131900060