Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F22%3A00569077" target="_blank" >RIV/68378041:_____/22:00569077 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/22:PU145056
Result on the web
<a href="https://www.mdpi.com/1422-0067/23/8/4236" target="_blank" >https://www.mdpi.com/1422-0067/23/8/4236</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms23084236" target="_blank" >10.3390/ijms23084236</a>
Alternative languages
Result language
angličtina
Original language name
Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation
Original language description
Collagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on cell interactions. The resulting scaffolds thus differed in grain size, changing from nanoscale to microscopic, and possessed diverse morphological characteristics and resorbability. The materials' biological action was shown on human bone marrow MSCs. Scaffold morphology was identified by SEM. Using viability test, qPCR, and immunohistochemical staining, we evaluated the biological activity of all of the materials. This study revealed that the most suitable scaffold composition for osteogenesis induction is collagen I foam with calcined hydroxyapatite with a pore size of 360 +/- 130 mu m and mean particle size of 0.130 mu m. The expression of osteogenic markers RunX2 and ColI mRNA was promoted, and a strong synthesis of extracellular protein osteocalcin was observed. ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
23
Issue of the periodical within the volume
8
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
4236
UT code for WoS article
000786142100001
EID of the result in the Scopus database
2-s2.0-85127855615