Characterization of human seminal plasma proteins homologous to boar spermadhesin AQN 1
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F04%3A00105279" target="_blank" >RIV/68378050:_____/04:00105279 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Characterization of human seminal plasma proteins homologous to boar spermadhesin AQN 1
Original language description
We immunobiochemically detected human seminal plasma proteins related to boar AQN 1 spermadhesin - hSA (human SpermAdhesin-like) proteins. In this study, we focused on further characterization of their 14kDa-, 16kDa- and 18kDa- forms (hSA-14, hSA 16 andhSA-18). Two hSA 14 isoforms interacted with heparin. Using indirect immunofluorescent microscopy, hSA protein epitopes were localized on the tail of human ejaculated sperm. On the basis of an ability of two isoforms of hSA proteins to interact with heparin and their localization on the sperm surface can be suggested that at least some of these proteins might be involved in sperm capacitation and fertilization in the human
Czech name
Charakterizace proteinů lidské semenné plazmy homologních s kančím spermadhezinem AQN 1
Czech description
Imunobiochemicky jsme určovali proteiny lidské semenné plazmy příbuzné kančímu spermadhezinu AQN 1 - proteiny hSA (human SpermAdhesin-like). V této studii jsme se zaměřili na další charakterizaci jejich forem o 14 kDa, 16 kDa a 18 kDa (hSA-14, hSA-16, hSA-18). Dvě izoformy hSA 14 reagovaly s heparinem. Pomocí nepřímé imunofluorescenční mikroskopie byly proteinové epitopy hSA lokalizovány na bičíku lidských ejakulovaných spermií. Na základě schopnosti obou izoforem proteinů hSA reagovat s heparinem a jejich lokalizaci na povrchu spermií lze vyvodit, že alespoň některé tyto proteiny by se mohly účastnit kapacitace spermií a oplodnění u člověka
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Biology of Reproduction
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
308
Publisher name
Society for the Study of Reproduction
Place of publication
Madison
Event location
Vancouver
Event date
Aug 1, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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