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Characterization of human seminal plasma proteins homologous to boar spermadhesin AQN 1

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F04%3A00105279" target="_blank" >RIV/68378050:_____/04:00105279 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Characterization of human seminal plasma proteins homologous to boar spermadhesin AQN 1

  • Original language description

    We immunobiochemically detected human seminal plasma proteins related to boar AQN 1 spermadhesin - hSA (human SpermAdhesin-like) proteins. In this study, we focused on further characterization of their 14kDa-, 16kDa- and 18kDa- forms (hSA-14, hSA 16 andhSA-18). Two hSA 14 isoforms interacted with heparin. Using indirect immunofluorescent microscopy, hSA protein epitopes were localized on the tail of human ejaculated sperm. On the basis of an ability of two isoforms of hSA proteins to interact with heparin and their localization on the sperm surface can be suggested that at least some of these proteins might be involved in sperm capacitation and fertilization in the human

  • Czech name

    Charakterizace proteinů lidské semenné plazmy homologních s kančím spermadhezinem AQN 1

  • Czech description

    Imunobiochemicky jsme určovali proteiny lidské semenné plazmy příbuzné kančímu spermadhezinu AQN 1 - proteiny hSA (human SpermAdhesin-like). V této studii jsme se zaměřili na další charakterizaci jejich forem o 14 kDa, 16 kDa a 18 kDa (hSA-14, hSA-16, hSA-18). Dvě izoformy hSA 14 reagovaly s heparinem. Pomocí nepřímé imunofluorescenční mikroskopie byly proteinové epitopy hSA lokalizovány na bičíku lidských ejakulovaných spermií. Na základě schopnosti obou izoforem proteinů hSA reagovat s heparinem a jejich lokalizaci na povrchu spermií lze vyvodit, že alespoň některé tyto proteiny by se mohly účastnit kapacitace spermií a oplodnění u člověka

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Biology of Reproduction

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    308

  • Publisher name

    Society for the Study of Reproduction

  • Place of publication

    Madison

  • Event location

    Vancouver

  • Event date

    Aug 1, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article