Colocalization of the tetraspanins, CO-029 and CD151, with integrins in human pancreatic adenocarcinoma: impact on cell motility

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00021319" target="_blank" >RIV/68378050:_____/05:00021319 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Colocalization of the tetraspanins, CO-029 and CD151, with integrins in human pancreatic adenocarcinoma: impact on cell motility

Original language description

The majority of pancreatic and colorectal tumors expressed the a2, a3, a6, b1, and b4 integrins and the tetraspanins CD9, CD63, CD81, CD151, and CO-029. Expression of a6b4 and CO-029 was restricted to tumor cells, whereas a1, a2, a3, a6, b1, and CD9, CD81, CD151 were also expressed by the surrounding stroma. CD63, CD81, and b1 expression was observed at comparably high levels in healthy pancreatic tissue. al3b1 frequently colocalized and coimmunoprecipitated with CD9, CD81, and CD151, whereas a6b4 colocalized and coimmunoprecipitated mostly with CD151 and CO-029. Notably, protein kinase C activation strengthened only the colocalization of CD151 and CO-029 with b4 and was accompanied by internalization of the integrin-tetraspanin complex, decreased laminin 5 adhesion, and increased cell migration. CONCLUSION: Integrin a6b4 is selectively up-regulated in pancreatic and colorectal cancer. The association of a6b4 with CD151 and CO-029 correlates with increased tumor cell motility.

Czech name

Společná lokalizace tetraspaninů CO-029 a CD151 s integriny u lidských adenokarcinomů slinivky: vliv na buněčnou motilitu

Czech description

Pankreatické a kolorektální tumory exprimují integriny a2, a3, a6, b1, b4 a tetraspaniny CD9, CD63, CD81, CD151, CO-029. Exprese a6b4 a CO-029 byla omezena na nádorové buňky, zatímco a1, a2, a3, a6, b1, and CD9, CD81, CD151 byly přítomny také na stromatu. CD63, CD81, a b1 byly přítomny na zdravé pankreatické tkáni. al3b1 kolokalizoval s CD9, CD81, a CD151, zatímco a6b4 s CD151 a CO-029. Aktivace PKC zesilovala kolokalizaci CD151 a CO-029 s b4 a byla doprovázena internalizací komplexu integrin-tetraspanin, sníženou adhezí k lamininu 5, a zvýšenou motilitou buněk. Závěr - Integrin a6b4 je selektivně up-regulován v pankreatických a kolorektálních tumorech. Asociace a6b4 s CD151 a CO-029 koreluje se zvýšenou motilitou nádorových buněk.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical Cancer Research

ISSN

1078-0432

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

2840-2852

UT code for WoS article

—

EID of the result in the Scopus database

—