Kit- and FceRI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00093983" target="_blank" >RIV/68378050:_____/08:00093983 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Kit- and FceRI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells

Original language description

Adaptor protein NTAL participates in signaling by FceRI and Kit in mast cells. It was found that whereas FceRI required Lyn and Syk for NTAL phosphorylation, Kit appeared to directly phosphorylate NTAL. Furthermore, co-transfection studies with NTAL constructs revealed that Lyn, Syk, and Kit phosphorylate different tyrosines in NTAL. The tyrosines principally phosphorylated by Syk were recognized as Grb2-binding sites, whereas Lyn and Kit phosphorylated other tyrosines, both inside and outside of thesemotifs. Pull down studies revealed that PLCg(1) associated with the two terminal Syk-phosphorylated Grb2-binding sites, which would help to explain the observed decrease in antigen-induced calcium signal and degranulation in NTAL-knock down-human mast cells. The observations reported herein indicate that NTAL may be differentially utilized by specific receptors for relaying alternative signals.

Czech name

Diferenciální fosforylace transmembránového adaptorového proteinu NTAL/LAB/LAT2 indukovaná receptory Kit a FceRI umožňuje flexibilitu jeho funkce v mastocytech

Czech description

Adaptorový protein NTAL se účastní signalizace pomocí receptorů FceRI a Kit v mastocytech. Zatímco fosforylace indukovaná FceRI vyžaduje kinasy Lyn a Syk, Kit fosforyluje NTAL přímo. Kotransfekční studie ukázaly, že Lyn, Syk a Kit fosforylují různé tyrosinové zbytky NTAL. Tyrosiny fosforylované pomocí Syk váží Grb2, zatímco Lyn a Kit fosforylují jiné tyrosiny. Imunoprecipitační experimenty ukázaly, že PLCg(1) asociuje se 2 terminálními Grb2-vazebnými místy fosforylovanými kinasou Syk, což by mohlo vysvětlit efekty potlačení exprese NTAL v lidských mastocytech. Tyto výsledky ukazují, že NTAL může být využíván různými způsoby odlišnými receptory.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/1M0506" target="_blank" >1M0506: Center of Molecular and Cellular Immunology</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular Signalling

ISSN

0898-6568

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

195-205

UT code for WoS article

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EID of the result in the Scopus database

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