dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00371756" target="_blank" >RIV/68378050:_____/12:00371756 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkr702" target="_blank" >http://dx.doi.org/10.1093/nar/gkr702</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkr702" target="_blank" >10.1093/nar/gkr702</a>

Result language

angličtina

Original language name

dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells

Original language description

Double-stranded RNA (dsRNA) can enter sequence-specific RNA interference (RNAi), sequence-independent interferon (IFN) response and editing by adenosine deaminases pathways. To study the fate of dsRNA in vivo, we used transgenic mice ubiquitously expressing from a promoter an mRNA with a long hairpin. The expressed dsRNA did not cause any developmental defects nor activated the IFN response, which was inducible only at high expression levels in cultured cells. dsRNA was poorly processed into siRNAs in somatic cells while robust RNAi effects were found in oocytes, suggesting that somatic cells lack some factor(s) facilitating siRNA biogenesis. Expressed dsRNA did not cause transcriptional silencing in trans. Analysis of RNA editing revealed an edited small fraction of long dsRNA. RNA editing did not prevent cytoplasmic localization nor processing into siRNAs. Thus, a long dsRNA structure is well tolerated in mammalian cells and is mainly causing a robust RNAi response in oocytes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F09%2F0085" target="_blank" >GA204/09/0085: RNA silencing and long dsRNA in mammalian cells</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

399-413

UT code for WoS article

000298733500043

EID of the result in the Scopus database

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