RNAi-based methods for gene silencing in mouse oocytes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00423294" target="_blank" >RIV/68378050:_____/13:00423294 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-62703-191-2" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-191-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-191-2" target="_blank" >10.1007/978-1-62703-191-2</a>

Result language
angličtina
Original language name
RNAi-based methods for gene silencing in mouse oocytes
Original language description
RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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