CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555394" target="_blank" >RIV/68378050:_____/21:00555394 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2073-4425/12/4/540" target="_blank" >https://www.mdpi.com/2073-4425/12/4/540</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/genes12040540" target="_blank" >10.3390/genes12040540</a>
Alternative languages
Result language
angličtina
Original language name
CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells
Original language description
RNA interference (RNAi) designates sequence-specific mRNA degradation mediated by small RNAs generated from long double-stranded RNA (dsRNA) by RNase III Dicer. RNAi appears inactive in mammalian cells except for mouse oocytes, where high RNAi activity exists because of an N-terminally truncated Dicer isoform, denoted Dicer(O). Dicer(O) processes dsRNA into small RNAs more efficiently than the full-length Dicer expressed in somatic cells. Dicer(O) is expressed from an oocyte-specific promoter of retrotransposon origin, which is silenced in other cell types. In this work, we evaluated CRISPR-based strategies for epigenetic targeting of the endogenous Dicer gene to restore Dicer(O) expression and, consequently, RNAi. We show that reactivation of Dicer(O) expression can be achieved in mouse embryonic stem cells, but it is not sufficient to establish a robust canonical RNAi response.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/GX20-03950X" target="_blank" >GX20-03950X: In vivo mammalian RNAi revival</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genes
ISSN
2073-4425
e-ISSN
2073-4425
Volume of the periodical
12
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
540
UT code for WoS article
000643040500001
EID of the result in the Scopus database
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