Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00440940" target="_blank" >RIV/68378050:_____/14:00440940 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.febslet.2014.09.014" target="_blank" >http://dx.doi.org/10.1016/j.febslet.2014.09.014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.febslet.2014.09.014" target="_blank" >10.1016/j.febslet.2014.09.014</a>

Result language

angličtina

Original language name

Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases

Original language description

Gene targeting in mice mainly employs homologous recombination (HR) in embryonic stem (ES) cells. Although it is a standard way for production of genetically modified mice, the procedure is laborious and time-consuming. This study describes targeting ofthe mouse Rosa26 locus by transcription activator-like effector nucleases (TALENs). We employed TALEN-assisted HR in zygotes to introduce constructs encoding TurboRFP and TagBFP fluorescent proteins into the first intron of the Rosa26 gene, and in this way generated two transgenic mice. We also demonstrated that these Rosa26-specific TALENs exhibit high targeting efficiency superior to that of zinc-finger nucleases (ZFNs) specific for the same targeting sequence. Moreover, we devised a reporter assay toassess TALENs activity and specificity to improve the quality of TALEN-assisted targeting. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEBS Letters

ISSN

0014-5793

e-ISSN

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Volume of the periodical

588

Issue of the periodical within the volume

21

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

3982-3988

UT code for WoS article

000344075400029

EID of the result in the Scopus database

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