The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F15%3A00455761" target="_blank" >RIV/68378050:_____/15:00455761 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.15252/embj.201490648" target="_blank" >http://dx.doi.org/10.15252/embj.201490648</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15252/embj.201490648" target="_blank" >10.15252/embj.201490648</a>

Result language

angličtina

Original language name

The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing

Original language description

Initiation of zygotic transcription in mammals is poorly understood. In mice, zygotic transcription is first detected shortly after pronucleus formation in 1-cell embryos, but the identity of the transcribed loci and mechanisms regulating their expression are not known. Using total RNA-Seq, we have found that transcription in 1-cell embryos is highly promiscuous, such that intergenic regions are extensively expressed and thousands of genes are transcribed at comparably low levels. Striking is that transcription can occur in the absence of defined core-promoter elements. Furthermore, accumulation of translatable zygotic mRNAs is minimal in 1-cell embryos because of inefficient splicing and 30 processing of nascent transcripts. These findings provide novel insights into regulation of gene expression in 1-cell mouse embryos that may confer a protective mechanism against precocious gene expression that is the product of a relaxed chromatin structure present in 1-cell embryos. The results a

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EMBO Journal

ISSN

0261-4189

e-ISSN

—

Volume of the periodical

34

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

1523-1537

UT code for WoS article

000355996900010

EID of the result in the Scopus database

—