Profilin connects actin assembly with microtubule dynamics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F16%3A00473129" target="_blank" >RIV/68378050:_____/16:00473129 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1091/mbc.E15-11-0799" target="_blank" >http://dx.doi.org/10.1091/mbc.E15-11-0799</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1091/mbc.E15-11-0799" target="_blank" >10.1091/mbc.E15-11-0799</a>
Alternative languages
Result language
angličtina
Original language name
Profilin connects actin assembly with microtubule dynamics
Original language description
Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin: actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization from microtubule-associated platforms possible. Microtubules are implicated in focal adhesion turnover, cell polarity establishment, and migration, illustrating the coupling between actin and microtubule systems. Here we demonstrate that profilin is functionally linked to microtubules with formins and point to formins as major mediators of this association. To reach this conclusion, we combined different fluorescence microscopy techniques, including superresolution microscopy, with siRNA modulation of profilin expression and drug treatments to interfere with actin dynamics. Our studies show that profilin dynamically associates with microtubules and this fraction of profilin contributes to balance actin assembly during homeostatic cell growth and affects microtubule dynamics. Hence profilin functions as a regulator of microtubule (+)-end turnover in addition to being an actin control element.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA16-25159S" target="_blank" >GA16-25159S: Calcium signaling to microtubules in activated mast cells</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology of the Cell
ISSN
1059-1524
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
15
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
2381-2393
UT code for WoS article
000380825800005
EID of the result in the Scopus database
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