Overexpression of TET dioxygenases in seminomas associates with low levels of DNA methylation and hydroxymethylation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00477929" target="_blank" >RIV/68378050:_____/17:00477929 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/17:43913000 RIV/00064173:_____/17:N0000041

Result on the web

<a href="http://dx.doi.org/10.1002/mc.22638" target="_blank" >http://dx.doi.org/10.1002/mc.22638</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mc.22638" target="_blank" >10.1002/mc.22638</a>

Result language

angličtina

Original language name

Overexpression of TET dioxygenases in seminomas associates with low levels of DNA methylation and hydroxymethylation

Original language description

Germ cell tumors and particularly seminomas reflect the epigenomic features of their parental primordial germ cells (PGCs), including genomic DNA hypomethylation and expression of pluripotent cell markers. Because the DNA hypomethylation might be a result of TET dioxygenase activity, we examined expression of TET1-3 enzymes and the level of their product, 5-hydroxymethylcytosine (5hmC), in a panel of histologically characterized seminomas and non-seminomatous germ cell tumors. Expression of TET dioxygenase mRNAs was quantified by real-time PCR. TET1 expression and the level of 5hmC were examined immunohistochemically. Quantitative assessment of 5-methylcytosine (5mC) and 5hmC levels was done by the liquid chromatography-mass spectroscopy technique. We found highly increased expression of TET1 dioxygenase in most seminomas and strong TET1 staining in seminoma cells. Isocitrate dehydrogenase 1 and 2 mutations were not detected, suggesting the enzymatic activity of TET1. The levels of 5mC and 5hmC in seminomas were found decreased in comparison to non-seminomatous germ cell tumors and healthy testicular tissue. We propose that TET1 expression should be studied as a potential marker of seminomas and mixed germ cell tumors and we suggest that elevated expression of TET dioxygenase enzymes is associated with the maintenance of low DNA methylation levels in seminomas. This anti-methylator phenotype of seminomas is in contrast to the CpG island methylator phenotype (CIMP) observed in a fraction of tumors of various types.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/NT14601" target="_blank" >NT14601: Syncytins as markers of germ line tumors</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Carcinogenesis

ISSN

0899-1987

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

1837-1850

UT code for WoS article

000404896500001

EID of the result in the Scopus database

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