Retroviral host range extension is coupled with Env-activating mutations resulting in receptor-independent entry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00486607" target="_blank" >RIV/68378050:_____/17:00486607 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1073/pnas.1704750114" target="_blank" >http://dx.doi.org/10.1073/pnas.1704750114</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1704750114" target="_blank" >10.1073/pnas.1704750114</a>
Alternative languages
Result language
angličtina
Original language name
Retroviral host range extension is coupled with Env-activating mutations resulting in receptor-independent entry
Original language description
The extent of virus transmission among individuals and species is generally determined by the presence of specific membrane-embedded virus receptors required for virus entry. Interaction of the viral envelope glycoprotein (Env) with a specific cellular receptor is the first and crucial step in determining host specificity. Using a well-established retroviral model-avian Rous sarcoma virus (RSV)-we analyzed changes in an RSV variant that had repeatedly been able to infect rodents. By envelope gene (env) sequencing, we identified eight mutations that do not match the already described mutations influencing the host range. Two of these mutations-one at the beginning (D32G) of the surface Env subunit (SU) and the other at the end of the fusion peptide region (L378S)-were found to be of critical importance, ensuring transmission to rodent, human, and chicken cells lacking the appropriate receptor. Furthermore, we carried out assays to examine the virus entry mechanism and concluded that these two mutations cause conformational changes in the Env variant and that these changes lead to an activated, or primed, state of Env (normally induced after Env interaction with the receptor). In summary, our results indicate that retroviral host range extension is caused by spontaneous Env activation, which circumvents the need for original cell receptor. This activation is, in turn, caused by mutations in various env regions.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
—
Volume of the periodical
114
Issue of the periodical within the volume
26
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"E5148"-"E5157"
UT code for WoS article
000404108400018
EID of the result in the Scopus database
—