MRE11 stability is regulated by CK2-dependent interaction with R2TP complex

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00487326" target="_blank" >RIV/68378050:_____/17:00487326 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10369098

Result on the web

<a href="http://dx.doi.org/10.1038/onc.2017.99" target="_blank" >http://dx.doi.org/10.1038/onc.2017.99</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/onc.2017.99" target="_blank" >10.1038/onc.2017.99</a>

Result language

angličtina

Original language name

MRE11 stability is regulated by CK2-dependent interaction with R2TP complex

Original language description

The MRN (MRE11-RAD50-NBS1) complex is essential for repair of DNA double-strand breaks and stalled replication forks. Mutations of the MRN complex subunit MRE11 cause the hereditary cancer-susceptibility disease ataxia-telangiectasia-like disorder (ATLD). Here we show that MRE11 directly interacts with PIH1D1, a subunit of heat-shock protein 90 cochaperone R2TP complex, which is required for the assembly of large protein complexes, such as RNA polymerase II, small nucleolar ribonucleoproteins and mammalian target of rapamycin complex 1. The MRE11-PIH1D1 interaction is dependent on casein kinase 2 (CK2) phosphorylation of two acidic sequences within the MRE11 C terminus containing serines 558/561 and 688/689. Conversely, the PIH1D1 phospho-binding domain PIH-N is required for association with MRE11 phosphorylated by CK2. Consistent with these findings, depletion of PIH1D1 resulted in MRE11 destabilization and affected DNA-damage repair processes dependent on MRE11. Additionally, mutations of serines 688/689, which abolish PIH1D1 binding, also resulted in decreased MRE11 stability. As depletion of R2TP frequently leads to instability of its substrates and as truncation mutation of MRE11 lacking serines 688/689 leads to decreased levels of the MRN complex both in ATLD patients and an ATLD mouse model, our results suggest that the MRN complex is a novel R2TP complex substrate and that their interaction is regulated by CK2 phosphorylation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA14-34264S" target="_blank" >GA14-34264S: Role of R2TP complex in DNA damage response and cell proliferation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncogene

ISSN

0950-9232

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

34

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

4943-4950

UT code for WoS article

000408234400010

EID of the result in the Scopus database

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