Structural and functional basis of mammalian microRNA biogenesis by Dicer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00567393" target="_blank" >RIV/68378050:_____/22:00567393 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/22:00128561
Result on the web
<a href="https://doi.org/10.1016/j.molcel.2022.10.010" target="_blank" >https://doi.org/10.1016/j.molcel.2022.10.010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molcel.2022.10.010" target="_blank" >10.1016/j.molcel.2022.10.010</a>
Alternative languages
Result language
angličtina
Original language name
Structural and functional basis of mammalian microRNA biogenesis by Dicer
Original language description
MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucle-ases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is spe-cifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer´s DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the com-plete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicerd???miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleav-age-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Cell
ISSN
1097-2765
e-ISSN
1097-4164
Volume of the periodical
82
Issue of the periodical within the volume
21
Country of publishing house
US - UNITED STATES
Number of pages
30
Pages from-to
"4064"-"4079e.13"
UT code for WoS article
000898565300011
EID of the result in the Scopus database
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