Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00644361" target="_blank" >RIV/68378050:_____/25:00644361 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10515580
Result on the web
<a href="https://rupress.org/jcb/article-abstract/224/8/e202309128/278087/Dynamic-interaction-of-spliceosomal-snRNPs-with?redirectedFrom=fulltext" target="_blank" >https://rupress.org/jcb/article-abstract/224/8/e202309128/278087/Dynamic-interaction-of-spliceosomal-snRNPs-with?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1083/jcb.202309128" target="_blank" >10.1083/jcb.202309128</a>

Result language
angličtina
Original language name
Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics
Original language description
The Cajal body (CB) is a conserved non-membrane nuclear structure where several steps of small nuclear RNP particle (snRNP) biogenesis take place. It has been proposed that CB formation follows a liquid-liquid phase separation model, but this hypothesis has never been rigorously tested. Here, we applied live-cell imaging to show that the key CB assembly factor coilin is mobile within the CB, and we revealed a diffusion barrier that limits the coilin exchange between CBs and the nucleoplasm. We generated single aa mutations and demonstrated that RNA-dependent coilin oligomerization and coilin interaction with snRNP are essential for CB formation and maintenance. We applied these data to formulate a mathematical model that links the movement of coilin within the nucleoplasm, CB, and across the boundary with its oligomerization and snRNP binding. Our results illustrate CB as a structure dynamically responding to snRNP assembly and recycling.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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