Beyond FoxP3-Identification of a Chicken Regulatory T Cell Signature
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00645080" target="_blank" >RIV/68378050:_____/25:00645080 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/eji.70106" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/eji.70106</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/eji.70106" target="_blank" >10.1002/eji.70106</a>
Alternative languages
Result language
angličtina
Original language name
Beyond FoxP3-Identification of a Chicken Regulatory T Cell Signature
Original language description
Regulatory T cells (Tregs), defined by the lineage-specific transcription factor FoxP3, are crucial for immune regulation and have been studied extensively in mammals. However, avian Tregs remain poorly characterized, leaving gaps in our understanding of their evolutionary conservation and unique features. In this study, we investigated the phenotype of chicken Tregs to define reliable markers for their identification and characterization. We analyzed CD4(+) splenocytes sorted into CD25(negative), CD25(low), and CD25(high) subpopulations using RNA sequencing. FOXP3 and other Treg-associated genes were expressed in both CD25(low) and CD25(high) populations, showing that CD25 expression alone is insufficient to distinguish chicken Tregs. To refine the marker profile, we evaluated additional markers, including CTLA-4 and GITR. Notably, we describe for the first time a chicken-specific CTLA-4 antibody, which uniquely stains CTLA-4 exclusively in intracellular (ic) compartments, distinguishing it from mammalian counterparts. Single-cell RNA sequencing further confirmed distinct FOXP3(+) clusters enriched for expression of CTLA4 and TNFRSF18 (encoding GITR). While CTLA-4's ic expression limits usability in functional assays, the combination of CD4(+)/CD25(+)/CTLA-4(+)/GITR(+) represents the most accurate characterization of putative chicken Tregs to date. These findings highlight evolutionary conservation and species-specific differences in Treg markers, providing the foundation for future studies on chicken Treg functionality.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Immunology
ISSN
0014-2980
e-ISSN
1521-4141
Volume of the periodical
55
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
e70106
UT code for WoS article
001651771900018
EID of the result in the Scopus database
2-s2.0-105025378652