Nanomolar hydrogen peroxide detection using horseradish peroxidase covalently linked to undoped nanocrystalline diamond surfaces
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F12%3A00384947" target="_blank" >RIV/68378271:_____/12:00384947 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21340/12:00200432
Result on the web
<a href="http://dx.doi.org/10.1021/la202967t" target="_blank" >http://dx.doi.org/10.1021/la202967t</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/la202967t" target="_blank" >10.1021/la202967t</a>
Alternative languages
Result language
angličtina
Original language name
Nanomolar hydrogen peroxide detection using horseradish peroxidase covalently linked to undoped nanocrystalline diamond surfaces
Original language description
In this article, we report on the low-level detection of hydrogen peroxide, a key player in the redox signaling pathway and a toxic product in the cellular system, using a colorimetric solution assay. Amine-terminated undoped nanocrystalline diamond thinfilms were grown on glass using a linear-antenna microwave plasma CVD process. The enzymatic activity of immobilized HRP was determined with a colorimetric test based on the HRP-catalyzed oxidation of 2,2-azino-bis(3-ethylbenzothiazoline-6-sufonic acid(ABTS) in the presence of hydrogen peroxide. The surface coverage of active HRP was estimated to be ? = 7.3 1013 molecules cm?2. The use of the functionalized diamond surface as an optical sensor for the detection of hydrogen peroxide with a detection limit of 35 nM was demonstrated.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BM - Solid-state physics and magnetism
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Langmuir
ISSN
0743-7463
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
587-592
UT code for WoS article
000298904900076
EID of the result in the Scopus database
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