Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00507904" target="_blank" >RIV/68378271:_____/19:00507904 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/19:00507904
Result on the web
<a href="https://doi.org/10.1021/acs.langmuir.8b02742" target="_blank" >https://doi.org/10.1021/acs.langmuir.8b02742</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.langmuir.8b02742" target="_blank" >10.1021/acs.langmuir.8b02742</a>
Alternative languages
Result language
angličtina
Original language name
Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing
Original language description
The mechanisms underlying cell protection from cryoinjury are not yet fully understood. Recent biological studies have addressed cryopreserved cell survival but have not correlated the cryoprotection effectiveness with the impact of cryoprotectants on the most important cell structure, the nucleus, and the freeze/thaw process. We identified changes of cell nuclei states caused by different types of cryoprotectants and associate them with alterations of the freeze/thaw process in cells. Namely, we investigated both higher-order chromatin structure and nuclear envelope integrity as possible markers of freezing and thawing processes. Moreover, we analysed in detail the relationship between nuclear envelope integrity, chromatin condensation, freeze/thaw processes in cells and cryopreservation efficiency for DMSO, glycerol, trehalose and antifreeze protein.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Langmuir
ISSN
0743-7463
e-ISSN
—
Volume of the periodical
35
Issue of the periodical within the volume
23
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
7496-7508
UT code for WoS article
000471728600021
EID of the result in the Scopus database
2-s2.0-85056124752