Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F20%3A00537260" target="_blank" >RIV/68378271:_____/20:00537260 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/20:00537260 RIV/00216224:14740/20:00118258
Result on the web
<a href="http://hdl.handle.net/11104/0314994" target="_blank" >http://hdl.handle.net/11104/0314994</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S1600576720013096" target="_blank" >10.1107/S1600576720013096</a>
Alternative languages
Result language
angličtina
Original language name
Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
Original language description
Electron crystallography of sub-micrometre-sized 3D protein crystals has emerged recently as a valuable field of structural biology. In meso crystallization methods, utilizing lipidic mesophases, particularly lipidic cubic phases (LCPs), can produce high-quality 3D crystals of membrane proteins (MPs). A major step towards realizing 3D electron crystallography of MP crystals, grown in meso, is to demonstrate electron diffraction from such crystals. The first task is to remove the viscous and sticky lipidic matrix that surrounds the crystals without damaging the crystals. Additionally, the crystals have to be thin enough to let electrons traverse them without significant multiple scattering.n
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Crystallography
ISSN
0021-8898
e-ISSN
1600-5767
Volume of the periodical
53
Issue of the periodical within the volume
Dec
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
1416-1424
UT code for WoS article
000595702500002
EID of the result in the Scopus database
2-s2.0-85097300651