Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F25%3A00618720" target="_blank" >RIV/68378271:_____/25:00618720 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11320/25:10513051 RIV/00023001:_____/25:00085381

Result on the web

<a href="https://hdl.handle.net/11104/0365605" target="_blank" >https://hdl.handle.net/11104/0365605</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/D5TB00074B" target="_blank" >10.1039/D5TB00074B</a>

Result language

angličtina

Original language name

Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake

Original language description

DNA nanostructures (DNs) have gained popularity in various biomedical applications due to their unique properties, including structural programmability, ease of synthesis and functionalization, and low cytotoxicity. Effective utilization of DNs in biomedical applications requires a fundamental understanding of their interactions with living cells and the mechanics of cellular uptake. Current knowledge primarily focuses on how the physicochemical properties of DNs, such as mass, shape, size, and surface functionalization, affect uptake efficacy. However, the role of cellular mechanics and morphology in DN uptake remains largely unexplored. In this work, we show that cells subjected to geometric constraints remodel their actin cytoskeleton, resulting in differential mechanical force generation that facilitates DN uptake. The length, number, and orientation of F-actin fibers are influenced by these constraints, leading to distinct mechanophenotypes. Overall, DN uptake is governed by F-actin forces arising from filament reorganisation under geometric constraints. These results underscore the importance of actin dynamics in the cellular uptake of DNs and suggest that leveraging geometric constraints to induce specific cell morphology adaptations could enhance the uptake of therapeutically designed DNs.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/LUAUS24038" target="_blank" >LUAUS24038: Mechanical regulation of functionalized DNA nanostructures processing by hepatic cells</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Materials Chemistry B

ISSN

2050-750X

e-ISSN

2050-7518

Volume of the periodical

13

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

2335-2351

UT code for WoS article

001400865800001

EID of the result in the Scopus database

2-s2.0-85215854455