CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F15%3A00301027" target="_blank" >RIV/68407700:21220/15:00301027 - isvavai.cz</a>
Result on the web
<a href="http://www.czech-in.org/ESB/ESB2015_Abstract_Book.pdf" target="_blank" >http://www.czech-in.org/ESB/ESB2015_Abstract_Book.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION
Original language description
Cell adhesion on different substrates as well as mechanical properties of cell membrane itself is one of the main topics in tissue engineering. Fluorescence microscope option for `state of the art’ nanomechanical test instruments enables clear visualization of Green Fluorescent Protein (GFP) transfected cells kept in a nutrient medium solution. However, identification of initial contact between an indenter probe and a very soft cell membrane can be very challenging. Contact forces are very low even compared to PBS meniscus forces and membrane adhesive forces acting on the probe. It has been recently shown that monitoring phase shift during oscillatory approach clearly identifies the contact point.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů