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CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F15%3A00301027" target="_blank" >RIV/68407700:21220/15:00301027 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.czech-in.org/ESB/ESB2015_Abstract_Book.pdf" target="_blank" >http://www.czech-in.org/ESB/ESB2015_Abstract_Book.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    CELL MEMBRANE STIFFNESS AND ADHESION MEASURED BY INSTRUMENTED NANOINDENTATION

  • Original language description

    Cell adhesion on different substrates as well as mechanical properties of cell membrane itself is one of the main topics in tissue engineering. Fluorescence microscope option for `state of the art’ nanomechanical test instruments enables clear visualization of Green Fluorescent Protein (GFP) transfected cells kept in a nutrient medium solution. However, identification of initial contact between an indenter probe and a very soft cell membrane can be very challenging. Contact forces are very low even compared to PBS meniscus forces and membrane adhesive forces acting on the probe. It has been recently shown that monitoring phase shift during oscillatory approach clearly identifies the contact point.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů