The effect of polymeric membrane surface on HaCaT cell properties
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F17%3A00312513" target="_blank" >RIV/68407700:21220/17:00312513 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.micron.2017.07.006" target="_blank" >https://doi.org/10.1016/j.micron.2017.07.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.micron.2017.07.006" target="_blank" >10.1016/j.micron.2017.07.006</a>
Alternative languages
Result language
angličtina
Original language name
The effect of polymeric membrane surface on HaCaT cell properties
Original language description
The control of the surface properties is an important issue for applicability of polymer membranes interacting with cells. In this work, the influence of surface roughness and stiffness of two polymer membranes on viability and mechanical properties of keratinocytes was studied. Terpolimer polyglicolide, polycaprolactone and polylactide, (PGA-PCL-PLA) and copolymer polycaprolactone, polyglicolide (PGA-PCL) substrates were used for membranes fabrication. Surface modification – the hydrolysis of the obtained membranes was carried out. The analysis of membranes’ surface properties revealed that RMS surface roughness and roughness factor of PGA-PCL-PLA membrane decreased after hydrolysis while its stiffness increased. In contrast, the PGA-PCL membrane stiffness was only slightly affected by NaOH treatment. Immortalized human keratinocytes (HaCaT) were grown under standard conditions on the surface of the studied membranes and characterized by means of atomic force microscopy and fluorescence microcopy. The results showed the substrate-dependent effect on cells’ properties.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Micron
ISSN
0968-4328
e-ISSN
1878-4291
Volume of the periodical
101
Issue of the periodical within the volume
October
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
162-169
UT code for WoS article
000413283300022
EID of the result in the Scopus database
2-s2.0-85026432759