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Processing Electrophoreogram Image

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F12%3A00201251" target="_blank" >RIV/68407700:21230/12:00201251 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Processing Electrophoreogram Image

  • Original language description

    Gel electrophoresis is one of the most frequently used methods in life-sciences research. Electrophoresis is a chemical separation method based on the effect of electric field on the particles. In this paper we present our proposed method for evaluatingthe results of gel electrophoresis images - electrophoreogram. The evaluation of the electrophoreogram images, i.e. to determine the molecular weight of divided particles, is commonly used commercial software. Our method segments image, automatically extracts the brightness curves across individual lanes, finds divided substances (bands) in these curves and calculates molecular weight of divided substances. We implemented the method and verified its success in comparison with commercial software on electrophoreogram images acquired using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the proposed method gives reliable results comparable with the evaluation using commercial software. Our method allow

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    BioDat 2012 - Conference on Advanced Methods of Biological Data and Signal Processing

  • ISBN

    978-80-01-05153-5

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

  • Publisher name

    České vysoké učení technické v Praze

  • Place of publication

    Praha

  • Event location

    Praha

  • Event date

    Nov 27, 2012

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article