Processing Electrophoreogram Image
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F12%3A00201251" target="_blank" >RIV/68407700:21230/12:00201251 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Processing Electrophoreogram Image
Original language description
Gel electrophoresis is one of the most frequently used methods in life-sciences research. Electrophoresis is a chemical separation method based on the effect of electric field on the particles. In this paper we present our proposed method for evaluatingthe results of gel electrophoresis images - electrophoreogram. The evaluation of the electrophoreogram images, i.e. to determine the molecular weight of divided particles, is commonly used commercial software. Our method segments image, automatically extracts the brightness curves across individual lanes, finds divided substances (bands) in these curves and calculates molecular weight of divided substances. We implemented the method and verified its success in comparison with commercial software on electrophoreogram images acquired using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the proposed method gives reliable results comparable with the evaluation using commercial software. Our method allow
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
BioDat 2012 - Conference on Advanced Methods of Biological Data and Signal Processing
ISBN
978-80-01-05153-5
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
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Publisher name
České vysoké učení technické v Praze
Place of publication
Praha
Event location
Praha
Event date
Nov 27, 2012
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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