In-vitro study of therapeutic radionuclides’ impact on selected tissue and tumour cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F19%3A00334661" target="_blank" >RIV/68407700:21340/19:00334661 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
In-vitro study of therapeutic radionuclides’ impact on selected tissue and tumour cell lines
Original language description
Beta emitters are widely used in treatment of various oncological diseases for a long time. Alpha emitters belong to new and perspective candidates for therapeutic use and some of them have been already introduced into clinical practise. Targeted alpha-particle therapy (TAT) is a rapidly evolving field of cancer treatment. Nevertheless, there are some severe issues that prevent TAT from being a leading modality in radionuclide therapy. The nuclear recoil effect that causes the daughter nuclei release from the original radiopharmaceuticals is a critical problem for alpha emitters. Moreover, targeting and proper dosimetry is still an issue. Therefore, we focused on the dosimetry on cellular and subcellular level with an aim to quantitatively and qualitatively compare the effect of alpha and beta emitters on living cells. For our study we used Ra-223, Sm-153 and Re-186 as a model radionuclides. All radionuclides were used in the range 0-8 kBq/mL. Studied cell lines were V79 (Chinese hamster lung fibroblasts), DU145 (human adenocarcinoma cell line) and U87 (human primary glioblastoma cell line) obtained from American Type Culture Collection (ATCC). All cell lines have been cultivated in the presence of Ra-223, Sm-153 or Re-186 for 24 hours after the monolayer of the cells was created. After the cultivation with Ra-223, Sm-153 or Re-186, the clonogenic survival test was performed and survival curves for all cell lines were constructed. All obtained survival curves correspond to the linearly quadratic model. Sensitivity of both human carcinoma cell lines (adenocarcinoma and glioblastoma cell line) to treatment by all used radionuclides is higher than the sensitivity of the Chinese hamster pulmonary fibroblast cell line. Sensitivity of tested carcinoma or tissue cell lines is higher to alpha treatment than to beta treatment using the same applicated activities of alpha or beta emitters.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
<a href="/en/project/NV16-30544A" target="_blank" >NV16-30544A: New multistage nanodiagnostics for cancer imaging and prediction of antiangiogenic therapy efficacy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů