Immobilization of concanavalin A lectin on a reduced graphene oxide-thionine surface by glutaraldehyde crosslinking for the construction of an impedimetric biosensor

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F17%3A63516601" target="_blank" >RIV/70883521:28110/17:63516601 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S157266571730259X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S157266571730259X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2017.04.019" target="_blank" >10.1016/j.jelechem.2017.04.019</a>

Result language
angličtina
Original language name
Immobilization of concanavalin A lectin on a reduced graphene oxide-thionine surface by glutaraldehyde crosslinking for the construction of an impedimetric biosensor
Original language description
Lectins, which are proteins with selective affinity to glycans or glycoproteins, have been recognized as promising agents for the construction of devices for the detection of specific glycoproteins and for glycoprofiling. This allows for the exploration of new potential biomarkers and for early diagnosis by detection of already known glycosylated biomarkers. In this work, immobilization of Concanavalin A (ConA) lectin on an electrochemically reduced graphene oxide (ErGO)/thionine (Thi) surface via glutaraldehyde (GA) crosslinking is investigated and applied for the impedimetric detection of the glycoprotein invertase (INV). An attachment of ConA/GA aggregates to the ErGO/Thi surface leads to a biosensor with a linear response in the concentration range of 10− 14–10− 8 mol for INV and a sensitivity of 6.1% of RCT change per decade of INV concentration. The sensitivity towards a negative control, i.e., INV with oxidized glycan moieties, is 2.97-fold lower than that towards INV. These findings provide a platform for the development of lectin-based, miniature and cheap biosensors for possible future disease diagnosis.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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