Genotypic and phenotypic detection of polyhydroxyalkanoate production in bacterial isolates from food
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63566578" target="_blank" >RIV/70883521:28110/23:63566578 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/24/2/1250" target="_blank" >https://www.mdpi.com/1422-0067/24/2/1250</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms24021250" target="_blank" >10.3390/ijms24021250</a>
Alternative languages
Result language
angličtina
Original language name
Genotypic and phenotypic detection of polyhydroxyalkanoate production in bacterial isolates from food
Original language description
Polyhydroxyalkanoates (PHAs) are widely used in medical and potentially in other applications due to their biocompatibility and biodegradability. Understanding PHA biosynthetic pathways may lead to the detection of appropriate conditions (substrates) for producing a particular PHA type by a specific microbial strain. The aim of this study was to establish a method enabling potentially interesting PHA bacterial producers to be found. In the study, all four classes of PHA synthases and other genes involved in PHA formation (fabG, phaA, phaB, phaG, and phaJ) were detected by PCR in 64 bacterial collection strains and food isolates. Acinetobacter, Bacillus, Cupriavidus, Escherichia, Klebsiella, Lelliottia, Lysinibacillus, Mammaliicoccus, Oceanobacillus, Pantoea, Peribacillus, Priestia, Pseudomonas, Rahnella, Staphylococcus, and Stenotrophomonas genera were found among these strains. Fructose, glucose, sunflower oil, and propionic acid were utilized as carbon sources and PHA production was detected by Sudan black staining, Nile blue staining, and FTIR methods. The class I synthase and phaA genes were the most frequently found, indicating the strains’ ability to synthesize PHA from carbohydrates. Among the tested bacterial strains, the Pseudomonas genus was identified as able to utilize all tested carbon sources. The Pseudomonas extremorientalis strain was determined as a prospect for biotechnology applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1661-6596
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
2
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
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UT code for WoS article
000950576100001
EID of the result in the Scopus database
2-s2.0-85146739846