Production of Polyhydroxyalkanoates in Bacterial Isolates from Food
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F23%3A63570118" target="_blank" >RIV/70883521:28110/23:63570118 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Production of Polyhydroxyalkanoates in Bacterial Isolates from Food
Original language description
Polyhydroxyalkanoates (PHAs) have extensive applications in the medical field and potentially in other sectors due to their biocompatibility and biodegradability. Gaining insights into the biosynthetic pathways of PHAs could facilitate the identification of optimal conditions (substrates) for producing specific types of PHAs using particular microbial strains. This research aimed to develop a methodology for identifying potentially valuable PHA-producing bacteria. Within this study, PCR analysis revealed the presence of all four classes of PHA synthases and other genes involved in PHA formation (fabG, phaA, phaB, phaG, and phaJ) in 64 bacterial collection strains and food isolates. The strains encompassed various genera, including Acinetobacter, Bacillus, Cupriavidus, Escherichia, Klebsiella, Lelliottia, Lysinibacillus, Mammaliicoccus, Oceanobacillus, Pantoea, Peribacillus, Priestia, Pseudomonas, Rahnella, Staphylococcus, and Stenotrophomonas. Carbon sources such as fructose, glucose, sunflower oil, and propionic acid were utilized, and PHA production was assessed through Sudan black staining, Nile blue staining, and FTIR techniques. Class I synthase and phaA genes were most frequently observed, indicating the strains' capacity for synthesizing PHA from carbohydrates. Among the bacterial strains tested, the Pseudomonas genus demonstrated the ability to utilize all carbon sources examined. Notably, the Pseudomonas extremorientalis strain exhibited potential for biotechnological applications.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů