Proteins and synthetic polymers blends for 3D printing of scaffolds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F25%3A63599259" target="_blank" >RIV/70883521:28110/25:63599259 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Proteins and synthetic polymers blends for 3D printing of scaffolds

Original language description

INTRODUCTION: Polymer blends of PCL, fibroin and PVDF were developed and processed using 3D printing to fabricate porous scaffolds. To enhance the specific surface area and promote cell adhesion1, phase separation techniques were applied to modify the surface topography of the printed structures. By combining synthetic and natural polymers, improved printability was achieved while preserving bioactive properties2. The resulting scaffolds demonstrate potential for applications in tissue engineering, particularly in guiding controlled cell interactions for regenerative medicine.EXPERIMENTAL/THEORETICAL STUDY: Blends of synthetic polymers (poly(ε-caprolactone) - PCL, poly(vinylidene-co-trifluoroethylene-co-chlorotrifluoroethylene) - P(VDFco-TrFE-co-CTFE)) with silk protein (SF) were prepared and processed using extrusion and electric field-assisted (EFA) 3D printing to fabricate porous scaffolds. Surface topography was modified through phase separation between polymer components or further enhanced by post-processing solvent treatments to increase specific surface area. The morphology and architecture of the structures were characterized using microscopy to evaluate the effects of material composition and processing on scaffold properties.RESULTS AND DISCUSSION: Scaffolds prepared by different techniques (Fig. 1) were surface-textured through phase separation induced by poor solvents (e.g., ethanol, DMSO). SF was added to the printing mixtures as a bioactive component, and it was found that SF also influenced the phase separation-induced change in surface topography (Fig. 2). Finally, the positive effect of the prepared structures on fibroblast cell spreading was observed (Fig. 3).CONCLUSION: New types of polymer blends based on synthetic polymers and proteins were designed for 3D printing of porous systems applicable in the field of regenerative medicine. Printed structures from such materials are characterized by complex architectures supporting basic cellular functions.REFERENCES:1. Vyas C. et al. , Materials Science and Engineering: C 2021, 118, DOI 10.1016/j.msec.2020.1114332. Majhy B. et al., RSC Advances 2021, 11, DOI 10.1039/D1RA02402G

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

<a href="/en/project/NU23-08-00243" target="_blank" >NU23-08-00243: Functional replacements for nerve tissue regeneration fabricated using advanced 3D printing techniques</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů