INJECTABLE COMPOSITE HYDROGEL FOR WOUND HEALING APPLICATIONS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F25%3A63595518" target="_blank" >RIV/70883521:28610/25:63595518 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

INJECTABLE COMPOSITE HYDROGEL FOR WOUND HEALING APPLICATIONS

Original language description

Injectable hydrogels are gaining prominence in biomedicine for applications such as tissue regeneration and wound healing [1]. Chitosan is a widely used biopolymer, but its poor solubility at physiological pH often necessitates chemical modifications that can introduce concerns about non-physiological degradation products [2].This study reports the development of novel injectable, self-healing, and anti-inflammatory composite hydrogels for tissue regeneration and wound healing, addressing the solubility limitations of chitosan. Water-soluble half-acetylated chitosan (SCN), synthesized by reacetylating chitosan to a degree of deacetylation (DD) of ~55% to ensure solubility at physiological pH without artificial functional groups, formed the hydrogel matrix. Dialdehyde cellulose (DAC), prepared via periodate oxidation of cellulose, was employed as a biocompatible crosslinker, uniquely binding SCN through dynamic Schiff base linkages and anchoring polypyrrole (PPy) nanoparticles via aldol condensation. PPy was incorporated for its established biocompatibility and therapeutic properties, including anti-inflammatory and wound healing-accelerating effects [3]. Hydrogels, also containing 5 wt% PPy colloids, were fabricated under mild conditions by mixing SCN and DAC solutions. Rheological analyses confirmed their injectable nature, characterized by shear-thinning behavior and rapid self-healing capabilities crucial for minimally invasive delivery. All formulations demonstrated excellent cytocompatibility with NIH/3T3 fibroblasts and RAW 264.7 macrophages. Notably, PPy-containing hydrogels significantly accelerated fibroblast migration in vitro, achieving up to two-fold faster wound closure compared to controls, indicating substantial wound healing promotion. Furthermore, these SCN-DAC-PPy hydrogels markedly reduced the production of pro-inflammatory mediators, nitric oxide (NO) and interleukin-6 (IL-6), by LPS-stimulated macrophages, highlighting their potent anti-inflammatory activity. Consequently, these composite hydrogels, with their combined injectability, self-healing capacity, biocompatibility, and immunomodulatory effects, hold significant promise as advanced biomaterials for wound care and tissue regeneration.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

<a href="/en/project/GA24-11534S" target="_blank" >GA24-11534S: Conductive (bio)polymer composites with covalently anchored polypyrrole for biomedical applications</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů