Development of high-resolution melting analysis for ABCB1 promoter methylation: clinical consequences in breast and ovarian carcinoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F19%3A00012659" target="_blank" >RIV/75010330:_____/19:00012659 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/19:43918352 RIV/00216208:11130/19:10393569 RIV/00216208:11140/19:10393569 RIV/00064173:_____/19:N0000152 RIV/00064203:_____/19:10393569
Result on the web
<a href="https://www.spandidos-publications.com/10.3892/or.2019.7186" target="_blank" >https://www.spandidos-publications.com/10.3892/or.2019.7186</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/or.2019.7186" target="_blank" >10.3892/or.2019.7186</a>
Alternative languages
Result language
angličtina
Original language name
Development of high-resolution melting analysis for ABCB1 promoter methylation: clinical consequences in breast and ovarian carcinoma
Original language description
Multidrug resistance to anticancer drugs, which is often associated with enhanced expression of the ATP-binding cassette (ABC) transporter P-glycoprotein (encoded by the ABCB1 gene) may limit the effects of cancer therapy. Epigenetic regulation of ABCB1 expression may thus have a clinical impact. A detailed assessment of ABCB1 promoter methylation is of importance for predicting therapy outcome and prognosis. Thus, validated methods for the analysis of ABCB1 promoter methylation are urgently required. In the present study, high-resolution melting (HRM) analysis of the CpG island regions covering the distal promoter of the ABCB1 gene was developed and compared with pyrosequencing. In addition, the clinical effects of the methylation status of the ABCB1 promoter were analyzed in patients with breast and ovarian carcinoma prior and subsequent to chemotherapy treatment. HRM analysis of ABCB1 methylation correlated with the results of pyrosequencing (P=0.001) demonstrating its analytical validity and utility. Hypermethylation of the analyzed ABCB1 promoter region was significantly correlated with low levels of the ABCB1 transcript in tumors from a subset of patients with breast and ovarian carcinoma prior to chemotherapy but not following treatment. Finally, high ABCB1 transcript levels were observed in tumors of patients with short progression-free survival prior to chemotherapy. Our data suggest the existence of functional epigenetic changes in the ABCB1 gene with prognostic value in tumor tissues of patients with breast and ovarian carcinoma. The clinical importance of such changes should be further evaluated.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oncology Reports
ISSN
1021-335X
e-ISSN
1791-2431
Volume of the periodical
42
Issue of the periodical within the volume
2
Country of publishing house
GR - GREECE
Number of pages
12
Pages from-to
763-774
UT code for WoS article
000475611600026
EID of the result in the Scopus database
2-s2.0-85068222348