Dissemination of the bla(CTX-M-15) gene among Enterobacteriaceae via outer membrane vesicles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F20%3A00013248" target="_blank" >RIV/75010330:_____/20:00013248 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jac/article/75/9/2442/5860391" target="_blank" >https://academic.oup.com/jac/article/75/9/2442/5860391</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dkaa214" target="_blank" >10.1093/jac/dkaa214</a>
Alternative languages
Result language
angličtina
Original language name
Dissemination of the bla(CTX-M-15) gene among Enterobacteriaceae via outer membrane vesicles
Original language description
Background: Bacterial outer membrane vesicles (OMVs) are an emerging source of antibiotic resistance transfer but their role in the spread of the bla(CTX-M-15) gene encoding the most frequent CTX-M ESBL in Enterobacteriaceae is unknown. Objectives: To determine the presence of bla(CTX-M-15) and other antibiotic resistance genes in OMVs of the CTX-M-15-producing MDR Escherichia coli O104:H4 outbreak strain and the ability of these OMVs to spread these genes among Enterobacteriaceae under different conditions. Methods: OMV-borne antibiotic resistance genes were detected by PCR. OMV-mediated transfer of bla(CTX-M-15) and the associated bla(TEM-1) was quantified under laboratory conditions, simulated intraintestinal conditions and under ciprofloxacin stress; resistance to antibiotics and the ESBL phenotype were determined by the CLSI disc diffusion methods and the presence of pESBL by plasmid profiling and Southern blot hybridization. Results: E. coli O104:H4 OMVs carried bla(CTX-M-15) and bla(TEM-1) located on the pESBL plasmid, but not chromosomal antibiotic resistance genes. The OMVs transferred bla(CTX-M-15), bla(TEM-1) and the associated pESBL into Enterobacteriaceae of different species. The frequencies of the OMV-mediated transfer were significantly increased under simulated intraintestinal conditions and under ciprofloxacin stress when compared with laboratory conditions. The 'vesiculants' (i.e. recipients that received the bla(CTX-M-15)-and bla(TEM-1)-harbouring pESBL via OMVs) acquired resistance to cefotaxime, ceftazidime and cefpodoxime and expressed the ESBL phenotype. They were able to further spread pESBL and the bla(CTX-M-15) and bla(TEM-1) genes via OMVs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Antimicrobial Chemotherapy
ISSN
0305-7453
e-ISSN
1460-2091
Volume of the periodical
75
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
2442-2451
UT code for WoS article
000574367100010
EID of the result in the Scopus database
2-s2.0-85089807470