Effects of 7-ketocholesterol on tamoxifen efficacy in breast carcinoma cell line models in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F23%3A00014249" target="_blank" >RIV/75010330:_____/23:00014249 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/23:43925817

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0960076023001097?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0960076023001097?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jsbmb.2023.106354" target="_blank" >10.1016/j.jsbmb.2023.106354</a>

Result language

angličtina

Original language name

Effects of 7-ketocholesterol on tamoxifen efficacy in breast carcinoma cell line models in vitro

Original language description

Oxysterols play significant roles in many physiological and pathological processes including cancer. They modulate some of the cancer hallmarks pathways, influence the efficacy of anti-cancer drugs, and associate with patient survival. In this study, we aimed to analyze the role of 7-ketocholesterol (7-KC) in breast carcinoma cells and its potential modulation of the tamoxifen effect. 7-KC effects were studied in two estrogen receptor (ER)positive (MCF-7 and T47D) and one ER-negative (BT-20) breast cancer cell lines. First, we tested the viability of cells in the presence of 7-KC. Next, we co-incubated cells with tamoxifen and sublethal concentrations of 7-KC. We also tested changes in caspase 3/7 activity, deregulation of the cell cycle, and changes in expression of selected genes/proteins in the presence of tamoxifen, 7-KC, or their combination. Finally, we analyzed the effect of 7-KC on cellular migration and invasion. We found that the presence of 7-KC slightly decreases the efficacy of tamoxifen in MCF-7 cells, while an increased effect of tamoxifen and higher caspase 3/7 activity was observed in the BT-20 cell line. In the T47D cell line, we did not find any modulation of tamoxifen efficacy by the presence of 7-KC. Expression analysis showed the deregulation in CYP1A1 and CYP1B1 with the opposite trend in MCF-7 and BT-20 cells. Moreover, 7-KC increased cellular migration and invasion potential regardless of the ER status. This study shows that 7-KC can modulate tamoxifen efficacy as well as cellular migration and invasion, making 7-KC a promising candidate for future studies.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30101 - Human genetics

Project

<a href="/en/project/NU22-08-00281" target="_blank" >NU22-08-00281: Multiomics for discovery of biomarkers for breast carcinoma resistance prediction</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Steroid Biochemistry and Molecular Biology

ISSN

0960-0760

e-ISSN

1879-1220

Volume of the periodical

232

Issue of the periodical within the volume

September

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

106354

UT code for WoS article

001037264900001

EID of the result in the Scopus database

2-s2.0-85163186728