The role of ubiquitin?proteasome pathway in the regulation of activity of two sperm surface proteins the aqn1 spermadhesin And the acrosin inhibitor in boar fertilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00394633" target="_blank" >RIV/86652036:_____/13:00394633 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The role of ubiquitin?proteasome pathway in the regulation of activity of two sperm surface proteins the aqn1 spermadhesin And the acrosin inhibitor in boar fertilization
Original language description
Problem. The AQN1 spermadhesin and acrosin inhibitor (AI) bind to the sperm plasma membrane at ejaculation. AQN1 has been implicated in sperm binding to zona pellucida (ZP) of the ovum as well as in sperm interactions with oviductal sperm reservoir. TheAI protects sperm from proteolytic degradation. The 26S proteasome is a multi-subunit protease specific to ubiquitinated substrate proteins. It is composed of a 20S proteasomal core with substrate degradation activity, and a 19S regulatory complex that acts in substrate recognition, deubiquitination, priming and transport to the 20S core. This study examined the role of two components of the 19S complex with, the ubiquitin C-hydrolase UCHL3 and PSMD8, in the AQN1-mediated boar sperm binding to ZP. Interaction of subunit PSMD4 with AI was detected. Results. Interaction of PSMD4 with AI provides evidence for the presence of cell proteasomes on the surface of male gametes. Detection of the ubiquitinated forms of AI supported the hypothesis
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP503%2F12%2F1834" target="_blank" >GAP503/12/1834: Identification of epigenetic biomarkers of male germ cell disorders linked to adverse environmental factors</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů