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Sperm surface protein AQ1 spermadhesin and ubiquitin-proteasome pathway in porcine anti-polyspermy defense

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00380390" target="_blank" >RIV/86652036:_____/12:00380390 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Sperm surface protein AQ1 spermadhesin and ubiquitin-proteasome pathway in porcine anti-polyspermy defense

  • Original language description

    Problem. One of the boar seminal plasma proteins called AQN1 spermadhesin binds to the sperm plasma membrane at ejaculation. This protein has been implicated in sperm binding to zona pellucid of the ovum as well as in sperm interactions with the epithelium of the oviductal sperm reservoir. The 26S proteasome is a multi-subunit protease specific to ubiquitinated substrate proteins. It is composed of a 20S proteasomal core with substrate degradation activity, and a 19S regulatory complex that acts in substrate recognition, deubiquitination, priming and transport to the 20S core. A high proteasome degradation activity and a high deubiquitinating activity were detected in the acrosome of boar spermatozoa. This study examined the role of two components of the 19S proteasome regulatory complex with deubiquitinating activity, ubiquitin C-terminal hydrolase UCHL3 and PSMD8, in the AQN1 spermadhesin-mediated boar sperm binding to zona pellucida. Results. We found that the activity and turnover

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů