Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F10%3A00346865" target="_blank" >RIV/86652036:_____/10:00346865 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization

Original language description

Proteolysis of ubiquitinated sperm and oocyte proteins by the 26S proteasome is necessary for mammalian fertilization. The study examined the role of PSMD4, a subunit of 19S regulatory complex responsible for proteasome-substrate recognition, in sperm-ZPpenetration during porcine fertilization in vitro (IVF). Immunofluorescence localized PSMD4 in the sperm acrosome. Immunoprecipitation and proteomic analysis revealed that PSMD4 co-precipitated with porcine sperm-associated acrosin inhibitor (AI). Ubiquitinated species of AI were isolated from boar sperm extracts by affinity purification of ubiquitinated proteins. Some proteasomes appeared to be anchored to the sperm head inner acrosomal membrane. In conclusion, the 19S regulatory complex subunit PSMD4is involved in the sperm-ZP penetration during fertilization. The recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for porcine/mammalian fertilization in vitro.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell and Tissue Research

ISSN

0302-766X

e-ISSN

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Volume of the periodical

341

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

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UT code for WoS article

000280593600010

EID of the result in the Scopus database

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