Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F21%3A00543280" target="_blank" >RIV/86652036:_____/21:00543280 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/21:00543280
Result on the web
<a href="https://doi.org/10.1002/smtd.202000985" target="_blank" >https://doi.org/10.1002/smtd.202000985</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/smtd.202000985" target="_blank" >10.1002/smtd.202000985</a>
Alternative languages
Result language
angličtina
Original language name
Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy
Original language description
Microtubules are cytoskeletal polymers of tubulin dimers assembled into protofilaments that constitute nanotubes undergoing periods of assembly and disassembly. Static electron micrographs suggest a structural transition of straight protofilaments into curved ones occurring at the tips of disassembling microtubules. However, these structural transitions have never been observed and the process of microtubule disassembly thus remains unclear. Here, label-free optical microscopy capable of selective imaging of the transient structural changes of protofilaments at the tip of a disassembling microtubule is introduced. Upon induced disassembly, the transition of ordered protofilaments into a disordered conformation is resolved at the tip of the microtubule. Imaging the unbinding of individual tubulin oligomers from the microtubule tip reveals transient pauses and relapses in the disassembly, concurrent with increased organization of protofilament segments at the microtubule tip. These findings show that microtubule disassembly is a discrete process and suggest a stochastic mechanism of switching from the disassembly to the assembly phas
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/ED2.1.00%2F19.0390" target="_blank" >ED2.1.00/19.0390: Introduction of new research methods to BIOCEV</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Small Methods
ISSN
2366-9608
e-ISSN
2366-9608
Volume of the periodical
5
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
2000985
UT code for WoS article
000611144400001
EID of the result in the Scopus database
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