Microtubules under mechanical pressure can breach dense actin networks
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00583048" target="_blank" >RIV/86652036:_____/23:00583048 - isvavai.cz</a>
Result on the web
<a href="https://journals.biologists.com/jcs/article/136/22/jcs261667/335502/Microtubules-under-mechanical-pressure-can-breach" target="_blank" >https://journals.biologists.com/jcs/article/136/22/jcs261667/335502/Microtubules-under-mechanical-pressure-can-breach</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/jcs.261667" target="_blank" >10.1242/jcs.261667</a>
Alternative languages
Result language
angličtina
Original language name
Microtubules under mechanical pressure can breach dense actin networks
Original language description
The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates microtubule organization within the cell, driven by the asymmetry of actin architecture along the cell periphery. The physical intertwining of these networks regulates spatial organization and force distribution in the microtubule network. Although their biochemical interactions are becoming clearer, the mechanical aspects remain less understood. To explore this mechanical interplay, we developed an in vitro reconstitution assay to investigate how dynamic microtubules interact with various actin filament structures. Our findings revealed that microtubules can align and move along linear actin filament bundles through polymerization force. However, they are unable to pass through when encountering dense branched actin meshworks, similar to those present in the lamellipodium along the periphery of the cell. Interestingly, immobilizing microtubules through crosslinking with actin or other means allow the buildup of pressure, enabling them to breach these dense actin barriers. This mechanism offers insights into microtubule progression towards the cell periphery, with them overcoming obstacles within the denser parts of the actin network and ultimately contributing to cell polarity establishment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cell Science
ISSN
0021-9533
e-ISSN
1477-9137
Volume of the periodical
136
Issue of the periodical within the volume
22
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
jcs261667
UT code for WoS article
001124512500003
EID of the result in the Scopus database
2-s2.0-85178536110