Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00583677" target="_blank" >RIV/86652036:_____/24:00583677 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/24:10482788 RIV/00216208:11310/24:10482788 RIV/00023001:_____/24:00084682
Result on the web
<a href="https://www.spandidos-publications.com/10.3892/br.2024.1738" target="_blank" >https://www.spandidos-publications.com/10.3892/br.2024.1738</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/br.2024.1738" target="_blank" >10.3892/br.2024.1738</a>
Alternative languages
Result language
angličtina
Original language name
Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases
Original language description
The current standard for graft rejection surveillance is endomyocardial biopsy (EMB), an invasive procedure with rare but potentially serious complications. Detection of circulating donor-derived cell-free DNA (ddcfDNA) is an option for noninvasive monitoring of graft injury and rejection. A 63-year-old man and a 65-year-old woman were monitored by EMB for allograft rejection. A total of 48 single-nucleotide polymorphisms with a minor allele frequency range of 0.4-0.5 were screened to distinguish donor and recipient DNA based on homozygosity, and digital droplet PCR was used to analyze ddcfDNA concentrations. Both subjects suffered rejection within the first 6 months after transplantation. The maximal ddcfDNA level of 270 copies (cp)/ml during EMB-confirmed acute cellular rejection (ACR, mild grade 1R/2, patient 1), and the maximal concentration of 1,846 cp/ml in the case of EMB-confirmed antibody-mediated rejection (AMR, grade 1+, patient 2), was detected. Individual monitoring of ddcfDNA dynamics from the 1st to the 6th month posttransplant reflected cardiac graft injury in patients suffering ACR or AMR, meaning that ddcfDNA may serve as a noninvasive biomarker.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NU20-06-00061" target="_blank" >NU20-06-00061: The possibility of using free circulating gDNA and mtDNA in the detection of graft rejection after heart transplantation.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedical Reports
ISSN
2049-9434
e-ISSN
2049-9442
Volume of the periodical
20
Issue of the periodical within the volume
3
Country of publishing house
GR - GREECE
Number of pages
7
Pages from-to
50
UT code for WoS article
001162521300001
EID of the result in the Scopus database
2-s2.0-85184431316