Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F25%3A00640258" target="_blank" >RIV/86652036:_____/25:00640258 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/25:10504130 RIV/00216208:11310/25:10504130 RIV/00064165:_____/25:10504130
Result on the web
<a href="https://www.nature.com/articles/s41467-025-63586-w" target="_blank" >https://www.nature.com/articles/s41467-025-63586-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-025-63586-w" target="_blank" >10.1038/s41467-025-63586-w</a>

Result language
angličtina
Original language name
Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation
Original language description
Mammalian oocytes are notoriously prone to chromosome segregation errors leading to aneuploidy. The spindle provides the machinery for accurate chromosome segregation during cell division. Mammalian oocytes lack centrioles and, therefore, mouse meiotic spindle relies on the organization of numerous acentriolar microtubule organizing centers into two poles (polar microtubule organizing centers, pMTOCs). The traditional view is that, in mammalian oocytes, microtubules are the sole cytoskeletal component responsible for regulating pMTOC organization and spindle assembly. We identify a previously unrecognized F-actin pool that surrounds pMTOCs, forming F-actin cage-like structure. We demonstrate that F-actin localization on the spindle depends on unconventional myosins X and VIIb. Selective disruption of spindle-localized F-actin, using myosin X/VIIb knockdown oocytes or photoswitchable Optojasp-1, perturbs pMTOC organization, leading to unfocused spindle poles and chromosome missegregation. Here, we unveil an important function of spindle-localized F-actin in regulating pMTOC organization, a critical process for ensuring the fidelity of meiotic spindle formation and proper chromosome segregation.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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