Physiological phenotyping of mammalian cell lines by enzymatic activity fngerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F19%3A00510085" target="_blank" >RIV/86652079:_____/19:00510085 - isvavai.cz</a>
Result on the web
<a href="https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-019-4697-y" target="_blank" >https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-019-4697-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13104-019-4697-y" target="_blank" >10.1186/s13104-019-4697-y</a>

Result language
angličtina
Original language name
Physiological phenotyping of mammalian cell lines by enzymatic activity fngerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study
Original language description
Objective Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, phosphofructokinase could be detected in samples of one or more mammalian cell lines. We characterized effects of sample dilution, assay temperature and repeated freeze–thaw cycles causing potential biases. After careful selection of experimental parameters, the presented semi-high throughput methods could be established as useful tool for physiological phenotyping of cultured mammalian cells.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
<a href="/en/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 – Development of the Centre of Global Climate Change Impacts Studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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